Zellkulturmedien GLYCOSTEM© BASAL GROWTH MEDIUM

ASAL GROWTH MEDIUM
Glycostem© Basal Growth Medium (“GBGM”) is an
animal-derived component free medium which contains only
human-derived or human recombinant proteins. GBGM is based on Iscove’s Modified Dulbecco’s Medium (“IMDM”). GBGM is an new and innovative basal growth medium for hematopoietic cells, including human hematopoietic stem and progenitor cells. Through the addition of specific components, such as cytokines, growth factors or human serum unprecedented culture conditions for human hematopoietic stem and progenitor cells, as well as other hematopoietic cells can be achieved.

Product features
GBGM has been specifically designed for the expansion and differentiation of hematopoietic stem and progenitor cells, such as CD34+ and CD133+ cells, in an animal-derived component free cell culture system.
A major feature of GBGM is, that it not only supports the expansion of cord blood (CB) CD34+ stem and progenitor cells, but also from bone marrow (BM) and G-CSF mobilised peripheral blood (mPB). [Figure 1]
GBGM has an improved effect on cell survival and only minimal cellular differentiation in an expansion and differentiation protocol, as compared to other serum free media. [Figure 2]
Therefore GBGM can be the basis for a very defined and pure generation of cellular products. [Figure 3]


Figure 1
Comparison of GBGM serum free medium for the expansion and differentiation of stem and progenitor cells with two other commercial available serum free media which are described to expand hematopoietic stem and progenitor cells.

CD34+ cells from cord blood were cultured and total cell expansion during five weeks was analysed. The results were normalized to the best medium, GBGM SCB. The compared serum free media SFM1 and SFM2 show only 20% of the expansion capacity than GBGM SCB.

Figure 2
A good cell survival, controlled cell differentiation and less cellular heterogeneity are major goals in the culture period
of hematopoietic stem and progenitor cells. Under defined culture conditions a cell population of interest is defined (“Live”-gate).

A | CD34+ cells from cord blood were cultured and cell survival and cellular homogeneity was analysed using Forward Scatter (FS) and Side Scatter (SS) plots to described the size and granularity of the cell population after four weeks of culture. In these experiments GBGM SCB shows 30-45% higher survival and homogeneity than SFM1 and SFM2.

B | CD34+ cells from bone marrow were cultured and cell survival and cellular homogeneity was analysed after six weeks of culture. In these experiments GBGM SBM shows 46-56% higher survival and homogeneity than SFM1 and SFM2.

Figure 3
CD 34+ cells from cord blood and bone marrow were first expanded and later differentiated into NK cell lineage.
The final product was analysed for the content of NK cells (CD56 positive cells).

In experiments using CD34+ cells from cord blood the highest purity and a significant higher NK cell content was seen for GBGM SCB after four weeks of culture than using SFM1 or SFM2.

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Zuletzt geändert am: Jun 24 2011 um 9:59 AM